A Mixture design study has been made to evaluate the colonic matrices of okra gum (OG) and Hypromellose using theophylline for combating nocturnal peak symptoms of asthma. As per design fourteen batches of matrices were prepared by wet granulation method and were evaluated for their physicochemical properties. In vitro dissolution was performed in simulated GI fluids viz. pH 1.2 (2h), pH 7.4 (3h), and pH 6.8 (1h) with or without rat caecal contents to check the integrity and release behavior of matrices. Analysis of variance was used to compute the statistical significance of quadratic model of the design. Physicochemical properties of matrices gave satisfactory results and as per I.P. specification. As per ANOVA there were no signs of any problems in our data and data points were almost linear. Formulations with higher amount OG and HPMC showed minimal drug release in upper GIT and were tested further for dissolution in presence of rat caecal contents. It was found that all batches releases its content within 3h of study in presence rat caecal content. These showed colon specific nature of okra gum and might be used as a carrier for chronotherapeutic delivery of theophylline in colon for the treatment of asthma.
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